abstract：:We have studied the effect of the adenylate cyclase-stimulating agent forskolin on expression of components of the plasminogen activation system in the human fibrosarcoma cell line HT-1080. By enzyme-linked immunosorbent assays, forskolin was found to cause a 2 to 4-fold decrease in intracellular and culture medium le...

journal_title：Molecular and cellular endocrinology

authors： Georg B,Riccio A,Andreasen P

更新日期：1990-08-20 00:00:00

abstract：:The aim of this study was to examine the first step in steroidogenesis in male and female gonads of fetal rats. Pregnenolone production was measured by radioimmunoassay in organ culture, conversion of [3H]cholesterol to [3H]pregnenolone was evaluated in isolated mitochondria and cytochrome P-450scc was revealed by imm...

journal_title：Molecular and cellular endocrinology

authors： Rouiller V,Gangnerau MN,Vayssiere JL,Picon R

更新日期：1990-08-20 00:00:00

abstract：:Thyroid hormones suppress transcription of the gene for the beta-subunit of thyrotropin (TSH beta). Since the TSH beta gene in both the mouse and the rat contains two start sites of transcription in exon 1, we have investigated whether expression of the gene from each start site is differentially regulated by thyroid ...

journal_title：Molecular and cellular endocrinology

authors： Gurr JA,Januszeski MM,Tidikis IM,Norcross JJ,Kourides IA

更新日期：1990-07-09 00:00:00

abstract：:Receptors for calcitriol are described in human decidua. They exhibit a dissociation constant of 35 +/- 6 pM and they are at concentrations similar to those found in other tissues (0.45 +/- 0.04 fmol/micrograms DNA). They are highly specific for calcitriol since neither of the other vitamin D3 derivatives nor the ster...

journal_title：Molecular and cellular endocrinology

authors： Delvin EE,Gagnon L,Arabian A,Gibb W

更新日期：1990-07-09 00:00:00

abstract：:Classical morphological studies of the folliculo-stellate (FS) cells of the anterior pituitary have suggested that these cells play roles as supporting cells, in metabolism and in macromolecular transport. Over the last 10 years the details of their activity in both trophic and catabolic processes has been clarified, ...

journal_title：Molecular and cellular endocrinology

authors： Allaerts W,Carmeliet P,Denef C

更新日期：1990-06-18 00:00:00

abstract：:Monoclonal antibodies generated to ovine prolactin were screened for their ability to neutralize the biological activity of prolactin from several species. By Western blot analysis, antibody 6F11 cross-reacted strongly with prolactin in homogenates of anterior pituitary glands from squirrel monkey, sheep and rat. In a...

journal_title：Molecular and cellular endocrinology

authors： Scammell JG,Wear LB,Von Haven R

更新日期：1990-06-18 00:00:00

abstract：:The addition of glucose to suspensions of HIT-T15 insulinoma cells caused a small, transient acidification followed by a gradual, progressive alkalinisation, as assessed by the fluorescent pH-sensitive dye 2',7'-biscarboxyethyl-5'-(6')-carboxyfluorescein (BCECF). Treatment of cells with acetate or lactate produced an ...

journal_title：Molecular and cellular endocrinology

authors： Trebilcock R,Lynch A,Tomlinson S,Best L

更新日期：1990-05-28 00:00:00

abstract：:The present report describes the initial characterization of a specific, high-affinity growth hormone binding protein (GH-BP) in adult male rat serum. GH-BP activity was measured by incubation of rat serum with [125I]hGH and [125I]rGH and separation of bound from free GH by dextran-coated charcoal. [125I]hGH binding t...

journal_title：Molecular and cellular endocrinology

authors： Amit T,Barkey RJ,Bick T,Hertz P,Youdim MB,Hockberg Z

更新日期：1990-04-17 00:00:00

abstract：:Lymphocyte cell lines were established from five patients with vitamin D-dependent rickets, type II (VDDR-II). These lines were established by infection with human T-lymphotrophic virus type I (HTLV-I). Binding of [3H]1 alpha,25-dihydroxyvitamin D3 (1,25(OH)2D3) to its receptor in these cell lines was compared to bind...

journal_title：Molecular and cellular endocrinology

authors： Koeffler HP,Bishop JE,Reichel H,Singer F,Nagler A,Tobler A,Walka M,Norman AW

更新日期：1990-03-26 00:00:00

abstract：:The conversion of androgens to estrogens is catalyzed by a complex of enzymes that includes a specific cytochrome P-450 aromatase (P-450arom). In this paper we describe the high level expression of aromatase activity in the rat Leydig cell tumor line, R2C. We also report the isolation of cDNA clones encoding the rat a...

journal_title：Molecular and cellular endocrinology

authors： Lephart ED,Peterson KG,Noble JF,George FW,McPhaul MJ

更新日期：1990-03-26 00:00:00

abstract：:The role of protein kinase C (PKC) in the mechanism of action of gonadotropin-releasing hormone (GnRH) upon gonadotropin secretion is controversial and therefore was investigated in primary cultures of rat anterior pituitary cells. A relatively selective PKC inhibitor, staurosporine, inhibited both GnRH- and 12-O-tetr...

journal_title：Molecular and cellular endocrinology

authors： Dan-Cohen H,Naor Z

更新日期：1990-03-05 00:00:00

abstract：:In this study we have investigated the protein phosphorylation pattern in the membrane fraction prepared from bovine luteal cells. The phosphorylation reaction was carried out in vitro, under defined conditions, using either [gamma-32P]ATP or [gamma-35S]ATP as the phosphate donor. The results obtained show that [gamma...

journal_title：Molecular and cellular endocrinology

authors： Budnik LT,Mukhopadhyay AK

更新日期：1990-03-05 00:00:00

abstract：:The human choriocarcinoma cell line, BeWo, synthesizes the glycoprotein hormone, human chorionic gonadotropin (hCG). We have undertaken this study to compare the synthesized and secreted forms of hCG and their alpha- and beta-subunits in cell cultures of BeWo cells to those forms of normal placental cells by immunobin...

journal_title：Molecular and cellular endocrinology

authors： Takeuchi Y,Sakakibara R,Ishiguro M

更新日期：1990-03-05 00:00:00

abstract：:The time- and dose-dependent effects of bovine activin A and bovine follicle stimulating hormone (FSH) suppressing protein (FSP) or follistatin on basal and FSH-induced steroidogenesis and inhibin production were studied in granulosa cells from immature, diethylstilbestrol (DES)-treated rats. In the presence of rat FS...

journal_title：Molecular and cellular endocrinology

authors： Xiao S,Findlay JK,Robertson DM

更新日期：1990-02-12 00:00:00

abstract：:Dispersed, estradiol (E2)-treated, rat pituitary cell cultures were used to examine the intracellular processing of progesterone (P) associated with its modulation of gonadotropin-releasing hormone (GnRH)-stimulated luteinizing hormone (LH) secretion. Enhancement and suppression of LH release was only observed with ac...

journal_title：Molecular and cellular endocrinology

authors： Krey LC,Kamel F,MacLusky NJ

更新日期：1990-01-22 00:00:00

abstract：:Cumulus enclosed or denuded oocytes obtained from ovaries of 25- to 27-day Sprague-Dawley rats underwent spontaneous germinal vesicle breakdown (GVBD) when cultured for 6 h in Krebs-Ringer's buffered solution (KRBS). This spontaneous division was found to be inhibited by adding beta-endorphin to the culture system and...

journal_title：Molecular and cellular endocrinology

authors： O WS

更新日期：1990-01-22 00:00:00

abstract：:The ontogenic developmental patterns of atrial natriuretic peptide (ANP) receptors of glomeruli and inner medullary collecting tubules (IMCT) were studied by measuring the specific binding of [125I]alpha-rat ANP 1-28 ([125I]alpha-RANP) to isolated glomeruli and IMCT microdissected from collagenase-treated kidneys of y...

journal_title：Molecular and cellular endocrinology

authors： Semmekrot B,Roseau S,Vassent G,Butlen D

更新日期：1990-01-02 00:00:00

abstract：:The effects of thyroid hormone on insulin-like growth factor I (IGF-I) production by Sertoli cells isolated from immature rats have been investigated. In Sertoli cells from hypothyroid rats the production of IGF-I was significantly lower than in controls and was greatly stimulated by the administration of triiodothyro...

journal_title：Molecular and cellular endocrinology

authors： Palmero S,Prati M,Barreca A,Minuto F,Giordano G,Fugassa E

更新日期：1990-01-02 00:00:00

abstract：:In this study we examined the mode of triiodothyronine (T3) and tetraiodothyronine (T4) transport in synaptosomal preparations from cerebral hemispheres of adult rat brain. Our results show that these hormones are transported by different mechanisms: T3 uptake is a saturable process and Hofstee analysis of the data re...

journal_title：Molecular and cellular endocrinology

authors： Kastellakis A,Valcana T

更新日期：1989-12-01 00:00:00

abstract：:A two-site immunoradiometric assay for insulin is described which is both rapid (processing time 60 min) and highly sensitive (lower detection limit 2 pM). Insulin is bound by a 125I-labelled mouse monoclonal antibody raised against human proinsulin and binding assessed by immunoprecipitation with an immunoadsorbent p...

journal_title：Molecular and cellular endocrinology

authors： Guest PC,Lowing C,Arden SD,Gray IP,Hutton JC

更新日期：1989-12-01 00:00:00

abstract：:The relationship between the structure of human growth hormone (hGH) and the hormone-receptor interaction has been investigated using as probes monoclonal antibodies (Mabs) to hGH of defined epitope specificity profile. Seven high affinity Mabs were studied for their ability (i) to inhibit the binding of 125I-hGH to N...

journal_title：Molecular and cellular endocrinology

authors： Strasburger CJ,Binder L,Elberg G,Cohen-Chapnik N,Kohen F,Gertler A

更新日期：1989-11-01 00:00:00

abstract：:Cardiovascular effects of parathyroid hormone (PTH) have been recently described. Pharmacological doses of PTH both reduce arterial pressure and increase blood flow of vascular beds. Two possible cellular mechanisms were investigated: (a) transmembrane Ca2+ fluxes and (b) cyclic AMP response in vascular smooth muscle....

journal_title：Molecular and cellular endocrinology

authors： Schleiffer R,Bergmann C,Pernot F,Gairard A

更新日期：1989-11-01 00:00:00

abstract：:While the transcriptional effects of glucocorticoid hormone manipulation on the pituitary pro-opiomelanocortin (POMC) gene have been documented, it is not yet clear whether glucocorticoids activate additional post-transcriptional mechanisms to regulate POMC gene expression. We have used RNA probes that span exon/intro...

journal_title：Molecular and cellular endocrinology

authors： Autelitano DJ,Blum M,Roberts JL

更新日期：1989-10-01 00:00:00

abstract：:Earlier studies indicate that rat hypothalamic oxytocin (OT) mRNA accumulation rises gradually during pregnancy and remains elevated throughout the lactation period. Here we show that, in addition, hypothalamic OT mRNA undergoes a structural change during this period. On gel electrophoresis, the size of rat OT mRNA in...

journal_title：Molecular and cellular endocrinology

authors： Zingg HH,Lefebvre DL

更新日期：1989-08-01 00:00:00

abstract：:We have examined the relationship between extracellular Ca2+, cytosolic free Ca2+ and insulin release in the clonal beta-cell line HIT-T15. Glucose-stimulated insulin release was dependent on the extracellular Ca2+ concentration in a dose-related manner; the threshold medium Ca2+ concentration for glucose-stimulated i...

journal_title：Molecular and cellular endocrinology

authors： Hughes SJ,Chalk JG,Ashcroft SJ

更新日期：1989-08-01 00:00:00

abstract：:It has already been shown that Wistar rat thyroid (WRT) cells in low concentrations of calf serum (0.5%) are under the influence of both thyrotropin (TSH) and insulin as regards growth. The present data show that epidermal growth factor (EGF), in concentrations up to 10 micrograms/ml, is not able to modify DNA synthes...

journal_title：Molecular and cellular endocrinology

authors： Rotella CM,Mavilia C,Frediani U,Toccafondi R

更新日期：1989-08-01 00:00:00

abstract：:The secretagogue effects of prolactin (PRL) and of various agents acting on cAMP levels, forskolin, cholera toxin and iloprost (a stable analogue of prostaglandin I2) have been assessed in lactating doe mammary gland fragments in vitro. Forskolin (10 microM), cholera toxin (1 microgram/ml) and iloprost (10 mM) stimula...

journal_title：Molecular and cellular endocrinology

authors： Ollivier-Bousquet M

更新日期：1989-08-01 00:00:00

abstract：:Peripheral benzodiazepine (BZ) binding sites (PBzS) were characterized on placental explant membranes. [3H]PK 11195, an isoquinoline carboxamide derivative, which is a ligand specific for PBzS, labeled these sites with an equilibrium dissociation constant of 2.1 nM; the maximal number of binding sites was 396 fmol/mg ...

journal_title：Molecular and cellular endocrinology

authors： Barnea ER,Fares F,Gavish M

更新日期：1989-07-01 00:00:00

abstract：:Glucocorticoid hormones suppress circulating concentrations of thyrotrophin (TSH), but their effect on synthesis of TSH in the pituitary gland is unclear. We have examined the influence of the glucocorticoid dexamethasone on serum TSH, pituitary TSH content and TSH beta- and alpha-subunit mRNA concentrations in pituit...

journal_title：Molecular and cellular endocrinology

authors： Ahlquist JA,Franklyn JA,Ramsden DB,Sheppard MC

更新日期：1989-06-01 00:00:00

abstract：:Ca2+-calmodulin-dependent protein phosphatase activity is found in cytoskeletons of Y-1 mouse adrenal and bovine fasciculata cells. The activity is inhibited by three inhibitors of calmodulin (trifluoperazine, W-7 and pimozide) with EC50 in the low micromolar range. Protein phosphatase activity is inhibited by vanadat...

journal_title：Molecular and cellular endocrinology

authors： Papadopoulos V,Brown AS,Hall PF

更新日期：1989-05-01 00:00:00

abstract：:Androgen-binding protein (ABP) is a testicular Sertoli cell secretory protein that acts as a carrier of androgen in the male reproductive tract. ABP has been characterized from a wide range of animal species, including man, rabbit and rat. However, it has been widely accepted that mice do not produce testicular ABP. W...

journal_title：Molecular and cellular endocrinology

authors： Wang YM,Sullivan PM,Petrusz P,Yarbrough W,Joseph DR

更新日期：1989-05-01 00:00:00

abstract：:In a model of pulmonary hypertension induced by a single injection of monocrotaline (MCT), we observed a time-dependent right ventricular hypertrophy, which became apparent in treated rats 21 days after administration of MCT and progressed through day 45. Associated with this right ventricular hypertrophy were time-de...

journal_title：Molecular and cellular endocrinology

authors： Oehlenschlager WF,Kurtz DT,Baron DA,Currie MG

更新日期：1989-04-01 00:00:00

abstract：:A kallikrein-related protease was purified from rat ventral prostate cytosol by means of DEAE-Sepharose chromatography, followed by gel filtration on Sephadex G-100 and CM-cellulose chromatography. Antibodies raised in rabbits against the purified protease recognize two bands on immunoblots of prostatic cytosol: a 31,...

journal_title：Molecular and cellular endocrinology

authors： Winderickx J,Swinnen K,Van Dijck P,Verhoeven G,Heyns W

更新日期：1989-04-01 00:00:00

abstract：:In two sublines of the estrogen receptor-positive human breast cancer cell line, MCF-7, propagated with 0.5% fetal calf serum (FCS) (subline 0.5) and without serum (subline 9), respectively, addition of 10% newborn calf serum (NCS) inhibits cell proliferation and stimulates the secretion of a 42 kDa protein. Both with...

journal_title：Molecular and cellular endocrinology

authors： Lykkesfeldt AE,Laursen I,Briand P

更新日期：1989-04-01 00:00:00

abstract：:Testicular peritubular cells produce paracrine mediators which modulate Sertoli cell function. The production of these mediators (P Mod-S) is controlled by androgens suggesting that mesenchymal-epithelial interactions play an important role in androgen action in the testis. We investigated whether mesenchymal cells fr...

journal_title：Molecular and cellular endocrinology

authors： Swinnen K,Cailleau J,Heyns W,Verhoeven G

更新日期：1989-03-01 00:00:00

abstract：:The regulation of the insulin receptor by cAMP has been examined in glial C6 cells. Incubation for 48 h with dibutyryl cyclic AMP produced a dose-dependent inhibition of 125I-insulin binding to the cells. Other agents that elevate intracellular cAMP levels such as forskolin and cholera toxin mimicked the effect of thi...

journal_title：Molecular and cellular endocrinology

authors： Montiel F,Aranda A,Pascual A

更新日期：1989-02-01 00:00:00

abstract：:The activity of the two cAMP-dependent protein kinases (PKa I and PKa II) was evaluated in dog thyroid cells in primary cultures after a 6-day growth period induced by either thyrotropin (TSH) or epidermal growth factor (EGF). Although the total PKa activity was not affected in cells cultured in the presence of TSH or...

journal_title：Molecular and cellular endocrinology

authors： Breton MF,Roger PP,Omri B,Dumont JE,Pavlovic-Hournac M

更新日期：1989-01-01 00:00:00

abstract：:Tumor necrosis factor-alpha (TNF-alpha) was found to induce type-1 plasminogen activator inhibitor (PAI-1) antigen in the human fibrosarcoma cell line HT-1080, and PAI-1 and urokinase-type plasminogen activator (u-PA) antigens in the human carcinoma cell line T-CAR1; tissue-type plasminogen activator (t-PA) antigen wa...

journal_title：Molecular and cellular endocrinology

authors： Georg B,Helseth E,Lund LR,Skandsen T,Riccio A,Danø K,Unsgaard G,Andreasen PA

更新日期：1989-01-01 00:00:00

abstract：:The expression of the gene coding for thyroperoxydase, the main enzyme involved in the synthesis of the thyroid hormones, is controlled by thyroid stimulating hormone. In vitro transcription assays performed on nuclei isolated from dog thyroid cells in primary culture showed that this control is at the transcription l...

journal_title：Molecular and cellular endocrinology

authors： Gerard CM,Lefort A,Libert F,Christophe D,Dumont JE,Vassart G

更新日期：1988-12-01 00:00:00

abstract：:Studies were performed to evaluate the relationships between the effects of angiotensin II on calcium metabolism and cytosolic free calcium concentration in primary monolayer cultures of bovine adrenal glomerulosa cells. As noted previously (Kramer (1988) Am. J. Physiol. (in press], angiotensin II produced rapid dose-...

journal_title：Molecular and cellular endocrinology

authors： Kramer RE

更新日期：1988-12-01 00:00:00